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Characterization of The Floral Meristem Identity gene LEAFY in M. quinquenervia

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Date Issued:
2018
Abstract/Description:
The study of flowering is an area of interest to agriculture due to the flower’s role in fruit formation and by the need to satisfy an increasing demand in food. Melaleuca quinquenervia is a plant species that has early and frequent flowering capabilities and understanding these may have important applications in agriculture. The floral meristem identity gene LEAFY (LFY) is essential for flowering in many plant species. The goal of this research is to clone and characterize the ortholog of LFY in M. quinquenervia. The first task was to screen a Melaleuca genomic library, consisting of bacterial colonies containing fragments of Melaleuca DNA. Screening was done by Polymerase Chain Reaction (PCR) using primers that were made based on the conserved sequences of LFY genes from other species. From the library, a bacterial colony was identified containing LFY sequences. If the colony contains the complete Melaleuca gene with homology to LFY (MqLFY-LIKE), we will be able to obtain the complete gene sequences. With the gene sequenced, primers can be made that will be utilized with PCR to reverse transcribe mRNA from floral buds to obtain the sequence of the MqLFY-LIKE protein.
Title: Characterization of The Floral Meristem Identity gene LEAFY in M. quinquenervia.
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Name(s): Tomas, Rodrigo, author
STEM Undergraduate Research Symposium
Type of Resource: text
Genre: Exhibition Posters
Issuance: single unit
Date Issued: 2018
Physical Form: PDF
Language(s): English
eng
Abstract/Description: The study of flowering is an area of interest to agriculture due to the flower’s role in fruit formation and by the need to satisfy an increasing demand in food. Melaleuca quinquenervia is a plant species that has early and frequent flowering capabilities and understanding these may have important applications in agriculture. The floral meristem identity gene LEAFY (LFY) is essential for flowering in many plant species. The goal of this research is to clone and characterize the ortholog of LFY in M. quinquenervia. The first task was to screen a Melaleuca genomic library, consisting of bacterial colonies containing fragments of Melaleuca DNA. Screening was done by Polymerase Chain Reaction (PCR) using primers that were made based on the conserved sequences of LFY genes from other species. From the library, a bacterial colony was identified containing LFY sequences. If the colony contains the complete Melaleuca gene with homology to LFY (MqLFY-LIKE), we will be able to obtain the complete gene sequences. With the gene sequenced, primers can be made that will be utilized with PCR to reverse transcribe mRNA from floral buds to obtain the sequence of the MqLFY-LIKE protein.
Identifier: fgcu_UGR_0101 (IID)
Note(s): Poster from the 2018 STEM Undergraduate Research Symposium. Research Mentor: Dr. Marilyn Cruz-Alvarez.
Subject(s): LEAFY
sequencing
PCR
Melaleuca
Polymerase chain reaction.
Persistent Link to This Record: http://purl.flvc.org/fgcu/fd/fgcu_UGR_0101
Use and Reproduction: Copyright held by author.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Owner Institution: FGCU

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