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Bioinformatic Annotation of ZenTime222 a Cluster T Mycobacteriophage

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Date Issued:
2016
Abstract/Description:
Bacteriophage are viruses that attach themselves to bacteria and inject their genome in order to hijack the cell’s machinery. ZenTime222 is a lytic bacteriophage isolated at Florida Gulf Coast University in 2013 that infects the host Mycobacterium smegmatis. Originally ZenTime222 was thought to be two separate phage, Zen222 and PriTime. However, during sequencing of the pooled genomes of all phage DNA found in the FGCU 2013 Virus Hunters class, it was discovered that these phage have identical genomes. ZenTime222 is a relatively unusual cluster T phage, with a genome length of 43,344 base pairs bp and 66 open reading frames, of which only 45% had known functions. Here we describe the genome architecture of ZenTime222 and highlight some of the interesting sequence elements discovered.
Title: Bioinformatic Annotation of ZenTime222 a Cluster T Mycobacteriophage.
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Name(s): Rosales, Kimberly A. , author
Tan, Amanda, author
Jenkins, Meagan, author
Czarneki, Karolina, author
MacMahon, author
STEM Undergraduate Research Symposium
Type of Resource: text
Issuance: single unit
Date Issued: 2016
Physical Form: PDF
Language(s): English
eng
Abstract/Description: Bacteriophage are viruses that attach themselves to bacteria and inject their genome in order to hijack the cell’s machinery. ZenTime222 is a lytic bacteriophage isolated at Florida Gulf Coast University in 2013 that infects the host Mycobacterium smegmatis. Originally ZenTime222 was thought to be two separate phage, Zen222 and PriTime. However, during sequencing of the pooled genomes of all phage DNA found in the FGCU 2013 Virus Hunters class, it was discovered that these phage have identical genomes. ZenTime222 is a relatively unusual cluster T phage, with a genome length of 43,344 base pairs bp and 66 open reading frames, of which only 45% had known functions. Here we describe the genome architecture of ZenTime222 and highlight some of the interesting sequence elements discovered.
Identifier: fgcu_UGR_0033 (IID)
Note(s): Poster from the 2016 STEM Undergraduate Research Symposium. Research Mentors: Dr. Scott Michael and Dr. Sharon Isern.
Subject(s): sequencing
Bacteriophage
Bioinformatics.
Persistent Link to This Record: http://purl.flvc.org/fgcu/fd/fgcu_UGR_0033
Use and Reproduction: In Copyright - Educational Use Permitted In Copyright - Educational Use Permitted
Owner Institution: FGCU

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