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Antibiotic resistance bacteria and the presence of the intI1 gene from class one integrons in recreational waters of Fort Myers

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Date Issued:
2017
Summary:
Integrons are versatile genetic units found in bacterial genomes that have evolved to code for antibiotic resistance. With overuse of antibiotics and poor waste management, aquatic environments have become a breeding ground for antibiotic resistant bacteria containing these integrons. It’s important to track integron activity to help prevent worldwide bacterial resistance. Water samples were taken in the Fort Myers area in triplicates from Dogs Beach, Fort Myers beach, FGCU waterfront, Yacht club, Barefoot Beach and 41 bridge. Descriptions of the area such as animals, people, vegetation and weather were observed at each location. Coliform bacteria were selected from the water samples using EMB agar and antibiotic resistance bacteria were isolated using various degrees of antibiotic agar. Bacterial isolates were sequenced via PCR, identified by agarose gel and compared to intl1 gene from a class one integron. Class one integrons confers antibiotic resistance and their presence is associated with poor waste management. The sample from Fort Myers beach had antibiotic resistant bacteria and the EMB had small traces of E. coli, but all bacterial isolates were negative for the present of the intl1 gene from the class one integron.
Title: Antibiotic resistance bacteria and the presence of the intI1 gene from class one integrons in recreational waters of Fort Myers.
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Name(s): Hollowell, Sidney, author
Fernandez, Gaby, author
Spring 2017 Senior Research in Biology and Environmental Studies
Type of Resource: text
Issuance: single unit
Date Issued: 2017
Physical Form: PDF
Language(s): English
eng
Summary: Integrons are versatile genetic units found in bacterial genomes that have evolved to code for antibiotic resistance. With overuse of antibiotics and poor waste management, aquatic environments have become a breeding ground for antibiotic resistant bacteria containing these integrons. It’s important to track integron activity to help prevent worldwide bacterial resistance. Water samples were taken in the Fort Myers area in triplicates from Dogs Beach, Fort Myers beach, FGCU waterfront, Yacht club, Barefoot Beach and 41 bridge. Descriptions of the area such as animals, people, vegetation and weather were observed at each location. Coliform bacteria were selected from the water samples using EMB agar and antibiotic resistance bacteria were isolated using various degrees of antibiotic agar. Bacterial isolates were sequenced via PCR, identified by agarose gel and compared to intl1 gene from a class one integron. Class one integrons confers antibiotic resistance and their presence is associated with poor waste management. The sample from Fort Myers beach had antibiotic resistant bacteria and the EMB had small traces of E. coli, but all bacterial isolates were negative for the present of the intl1 gene from the class one integron.
Identifier: fgcu_UGR_0032 (IID)
Note(s): Posters from senior research in biology and environmental studies - Spring 2017. Research mentor: Dr. Kathryne Byrne-Bailey.
Subject(s): Integrons
Antibiotic Resistance
recreational waters
Antibiotics.
Florida
Persistent Link to This Record: http://purl.flvc.org/fgcu/fd/fgcu_UGR_0032
Use and Reproduction: In Copyright - Educational Use Permitted In Copyright - Educational Use Permitted
Owner Institution: FGCU

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