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Variable Inhibition of Zika Virus Replication by Different Wolbachia Strains in Mosquito Cell Cultures

Title: Variable Inhibition of Zika Virus Replication by Different Wolbachia Strains in Mosquito Cell Cultures.
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Name(s): Schultz, Michaela J., author
Isern, Sharon, author
Michael, Scott F., author
Corley, Ronald B., author
Connor, John H., author
Frydman, Horacio M., author
Diamond, Michael S., editor
Type of Resource: text
Genre: Article
Date Issued: 2017-04-26
Language(s): English
Abstract: Mosquito-borne arboviruses are a major source of human disease. One strategy to reduce arbovirus disease is to reduce the mosquito's ability to transmit virus. Mosquito infection with bacterial endosymbiont, Wolbachia pipientis wMel, is a novel strategy to reduce Aedes mosquito competency for flavivirus infection. However, experiments investigating cyclic environmental temperatures have shown a reduction in maternal transmission of wMel potentially weakening the integration of this strain into a mosquito population relative to other Wolbachia strains. Consequently, it is important to investigate additional Wolbachia strains. All Zika virus (ZIKV) suppression studies are limited to the wMel Wolbachia strain. Here we show ZIKV inhibition by two different Wolbachia strains: wAlbB (isolated from Aedes albopictus mosquitoes) and wStri (isolated from the planthopper Laodelphax striatellus) in mosquito cells. The Wolbachia strain wStri inhibited ZIKV most effectively. Single-cycle infection experiments showed that ZIKV RNA replication and nonstructural protein 5 translation were reduced below the limits of detection in wStri-containing cells, demonstrating early inhibition of virus replication. ZIKV replication was rescued when Wolbachia was inhibited with a bacteriostatic antibiotic. We observed a partial rescue of ZIKV growth when Wolbachia infected cells were supplemented with cholesterol-lipid concentrate, suggesting competition for nutrients as one of the possible mechanisms of Wolbachia inhibition of ZIKV. Our data show that wAlbB and wStri infection causes inhibition of ZIKV making them attractive candidates for further in vitro mechanistic and in vivo studies and future vector-centered approaches to limit ZIKV infection and spread.
Identifier: 10.1128/JVI.00339-17 (doi), fgcu_ir_000053 (IID), http://jvi.asm.org/lookup/doi/10.1128/JVI.00339-17 (uri)
Subject(s): Zika virus
Wolbachia
mosquito
ZIKV
Persistent Link to This Record: http://purl.flvc.org/fgcu/flvc_fgcu_islandoraimporter_10.1128_JVI.00339-17_1519241224
Use and Reproduction: publisher.
Use and Reproduction: publisher
Owner Institution: FGCU
Is Part Of: Journal of Virology.
0022-538X